Table 4.
Gene | Expression | Fold-change ± SEM | Range | P value |
---|---|---|---|---|
Cdh5 | Endothelial | 0.08 ± 0.01 | 0.074-0.095 | <7.4 × 10−5 |
Tek | Endothelial | 0.002 ± 0.001 | 0.007-0.010 | <2.1 × 10−5 |
Vcam1 | Endothelial | 0.003 ± 0.004 | 0.001-0.009 | <3.2 × 10−3 |
Vwf | Endothelial/megakaryocyte | 248.92 ± 15.56 | 233.2-264.9 | <3.3 × 10−9 |
F5 | Hepatic/megakaryocyte | 73.41 ± 3.15 | 70.3-76.6 | <1.5 × 10−5 |
F8 | ? | 0.02 ± 0.01 | 0.011-0.029 | <1.5 × 10−4 |
To evaluate the potential contribution of blood contamination to transcript levels of endothelial-specific genes, the tissue lysates of kidney samples were set as a reference and the ΔCt values of individual blood samples (n = 4) were related to the mean ΔCt of the reference group.