Figure 2.

Runx1bEx6⁻ and Runx1bEx6e have higher colony-forming ability than full-length Runx1bEx6⁺. Lineage-negative BM cells were infected with indicated retroviral constructs. After 1-week selection in 1 μg/mL puromycin, 1 × 10⁵ cells were seeded into methylcellulose in duplicate. One week later, (A) colony counts and (B) cell counts per plate were evaluated. Mean ± SD of 5 independent experiments is shown. (C) Flow cytometric analysis of colony cells. The gating of CD11b-negative/low, -medium, and -high (from bottom to top) is shown in the plots. Representative result of 3 independent experiments is shown. (D) Quantification of the results in panel C. Mean ± SD of 3 independent experiments is shown.