Table 1.

Primer designs

Gene	Accession no.	Sequence
HLA-DRA	NM_019111	5′-GATGCTCCAAGCCCTCTCCCAG-3′ (probe) (22)
		5′-GCCTCTTCTCAAGCACTGGGA-3′ (sense) (21)
		5′-CCACCAGACCCACAGTCAGG-3′ (antisense) (20)
HLA-DMA	NM_006120	5′-TCCCTGAAGCTCCTACTCCAA-3′ (probe) (22)
		5′-CTGTGTGGCAAGAAGGTATG-3′ (sense) (20)
		5′-TCCTGGCAGTACACTGTGT-3′ (antisense) (19)
HLA-DMB	NM_002118	5′-GAGCAGGTGGCTTCGTGGC-3′ (probe) (19)
		5′-CATCTTTACAGAGCAGAGCAT-3′ (sense) (21)
		5′-ATGTGAAATCCTTTGGAGTCC-3′ (antisense) (21)
CIITA	NM_000246	5′-CTCAGAACCCGACACAGACAC-3′ (probe) (21)
		5′-CCTGGCTGGAGAAGAAGAG-3′ (sense) (19)
		5′-TCCTGGAAGACATACTGGTC-3′ (antisense) (20)
CD74	NM_004355	5′-CCAGCGAGGAGCAGAGTCAC-3′ (probe) (20)
		5′-TTATCTCCAACAATGAGCAACT-3′ (sense) (22)
		5′-ACAGGAAGTAGGCGGTGGT-3′ (antisense) (19)
HPRT1	NM_000194.2	5′-CAAGTTTGTTGTAGGATATGCCC-3′ (probe) (23)
		5′-CCAAAGATGGTCAAGGTCGC-3′ (sense) (20)
		5′-GACACAAACATGATTCAAATCC-3′ (antisense) (22)
PPIB	NM_000942.4	5′-GGTGAGCATGGCCAACGCAGG-3′ (probe) (22)
		5′-GGAGATGGCACAGGAGGAAAGA-3′ (sense) (22)
		5′-GGGAGCCGTTGGTGTCTTTG-3′ (antisense) (20)

Design of primers used for the messenger RNA quantification of major histocompatibility class II related genes and selected housekeeping genes using real-time polymerase chain reaction. CD74, HLA-DR antigen-associated invariant chain; CIITA, class II transactivator; HLA-DR/DM, human leukocyte antigen-DR/DM; HPRT1, hypoxanthine phosphoribosyltransferase 1; PPIB, peptidylprolyl isomerase B (cyclophilin B).