Figure 2.

Overexpression of miR-126 decreases invasion in J82 and EJ138 bladder cancer cell lines. (A) Validation of miR-126 overexpression in J82_FL and EJ138_FL cells stably infected with miR-126 by qRT–PCR. Effect of miR-126 overexpression in J82_FL and EJ138_FL on (B) cell proliferation (C and D) cell invasion and (E) cell apoptosis. Invasion of FL_126 cells is calibrated to the FL line (C). Invaded cells are stained with DAPI (D). Expression levels are calculated as log₂ fold change. *P<0.05, **P<0.005, and ***P<0.0005 by Student's t-test.