Fig. 2.

Effect of TVX on TopIIα activity. A reaction mixture containing kDNA in the absence (1st lane on the left) or presence (all other lanes) of TopIIα was incubated with 0 (VEH), 10, 30, 100, or 300 μM TVX or 10 μM VP-16. After 30 minutes, the reaction was quenched, and samples were separated on a 1% agarose gel and stained with ethidium bromide to visualize DNA decatenate migration. Image is representative from an n = 3.