Fig. 6.
Activation of NF-κB pathway by proinflammatory cytokines. Longitudinal muscle cells isolated from colon of control and TNBS-treated mice or from muscle strips cultured in the absence (control) or presence of IL-1β (10 ng/ml) or TNF-α (1 nM) for 24 hours were used for measurement of p65 subunit phosphorylation (i.e., activation) by Western blot using antibody to phospho-specific (Ser177/181) antibody (A) and IκBα degradation using antibody to IκBα (B). Western blot of the β-actin protein is shown for a control loading. Values are means ± S.E.M. of four experiments **P < 0.01, significantly different from control.