Fig. 7.

Cytokine-induced phosphorylation of AMPK at Ser⁴⁸⁵ by PKA derived from NF-κB activation. Longitudinal muscle cells were isolated from colon of control and TNBS-treated mice or from muscle strips cultured in the absence (control) or presence of IL-1β (10 ng/ml) or TNF-α (1 nM) for 24 hours (A). In some experiments, muscle strips were incubated with IL-1β in the presence of inhibitors of NF-κB pathway (MG-132, 10 µM) or PKA (myristoylated PKI, 1 µM) (B). Phosphorylation of AMPK at Ser⁴⁸⁵ was measured using phospho-specific antibody. Values are means ± S.E.M. of four experiments. **P < 0.01, significantly different from control.