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. 2014 Jul;96(1):93–103. doi: 10.1189/jlb.2A0713-393RR

Figure 3. TLR7, -9, and -3 ligands up-regulate Trex1 expression in cDCs in a STAT1-, STAT2-, and IFNAR1-mediated manner.

Figure 3.

We stimulated cDCs from B6 (A), IFNARKO (B), STAT2KO and STAT1KO (C), and appropriate WT mice with R848 1 μg/ml, CpG 10 μg/ml, and PolyI:C (PI:C) 200 ng/ml for 1 and 6 h and analyzed Trex1 and IFN-β RNA by qRT-PCR. Results of qRT-PCR were normalized to cyclophilin, and mean ± se of three (A), four (B), and three (C) independent experiments are shown. One representative experiment is shown for STAT1KO in C. (D and E) Protein expression of Trex1 in WT, STAT1KO, and STAT2KO cDCs stimulated with the same TLR ligands. Western blotting results were normalized to β-actin, and one representative blot and mean ± se from three independent experiments are shown in D and E.