Figure 3. Monocytes expressing high levels of CD163 are more susceptible to HIV infection.

(A) Monocytes were sorted based on expression level of CD163 using a MoFlo XDP (Beckman Coulter) cell sorter and infected with HIV-BaL, 4 h later. Cell purity was checked after each cell-sorting experiment and found >95%. (B) HIV-GAG normalized to GAPDH assayed by real-time RT-PCR. Average results of three independent experiments are presented as mean ± sd. *P < 0.01 CD163^high versus CD163^low.