Figure 2. Histamine increases MDSC survival and proliferation.

(A) Survival assessed by trypan blue exclusion over time course of MDSCs alone (circle) or with histamine (triangle). (B and C) Proliferation assays of MDSCs cultured alone, with histamine (H), or in combination with indicated agents, as measured by counts per minute (CPM). (D) MDSCs were cultured with histamine or media. *P < 0.05; **P < 0.005; ***P < 0.0005. Mean ± sd; n ≥ 10/group and at least two independent experiments.