Figure 3. Histamine influences MDSC gene expression.
Ly6C+ or Ly6G+ MDSCs were cultured ± histamine and analyzed (48 h) for Arg1 (A) or iNOS (B) message. Fold change of histamine-treated/media alone (A and B). Fold change of IL-13 and IL-4 message from histamine-treated Ly6G+/Ly6C+ cells (C). After normalization to GAPDH, data were analyzed using the ΔΔCt method.***P < 0.0005. Mean ± sd; n ≥ 5 from two experiments.