Figure 3.

Connexon activity of WT mouse fibroblasts (A), S3 transgenic mouse (B) and S3 incubated with KN93 (C), were incubated with LY for 2 h and examined under a fluorescent microscope. (D) Connexon activity in fibroblasts from WT, S3, and S3 treated with KN93 was monitored in a 96 well plate assay, using lucifer yellow as a fluorescent dye (see methods). Fluorescence is in arbitrary units. (E) Sciatic nerves, isolated either from Wild type mice (WT) or from S3 transgenic mice, have been cultured for 24 h without or with KN93, a CamKII inhibitor. Connexon activity has been evaluated using internalization of LY (see Methods). ^***p-value < 0.001.