Table 3.
FOCAL designed experiments for reactions in galacturonate and glucuronate catabolism
| Chosen reactiona | Enzyme | Associated gene | FOCAL selected carbon source |
|---|---|---|---|
galur  tagur |
D-Galacturonate isomerase | uxaC | D-Galacturonate |
| h + nadh + tagur altrn +
nad |
Altronate oxidoreductase | uxaB | D-Galacturonate |
| altrn → 2ddglcn + h2o | Altronate hydrolase | uxaA | D-Galacturonate |
| glcur fruur |
D-Glucuronate isomerase | uxaC | D-Glucuronate |
| fruur + h + nadh mana + nad |
D-Mannonate oxidoreductase | uxuB | D-Glucuronate |
| mana → 2ddglcn + h2o | D-Mannonate hydrolyase | uxuA | D-Glucuronate |
aAbbreviations match those shown in Figure 5.