Figure 8.

Hyperglycemia increases protein carbonyl modifications in the diaphragm. A) is a representative oxyblot in diaphragm homogenates from the four experimental groups. Image was obtained from the same gel, but lanes were not adjacent and are demarcated by the lines within the representative image. As shown, hyperglycemia produced multiple protein carbonyl modifications of diaphragm proteins. B) is the mean ± SEM of the total lane densitometry of the four experimental groups (n = 4). Administration of PEG-SOD, but not denatured PED-SOD, largely abolished these modifications indicating that excessive superoxide generation mediates this process. (P <0.001, *significantly different when compared to control or hyperglycemia + PEG-SOD). PEG-SOD, polyethylene glycol superoxide dismutase; SEM, standard error of the mean.