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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Biomed Mater Res A. 2013 Jun 4;102(4):1017–1025. doi: 10.1002/jbm.a.34764

Figure 1.

Figure 1

Generation of decellularized tissue scaffolds from mouse livers. (A) The key steps for decelluarizing mouse livers involve in (a) exposing the mouse abdomen, (b) identifying the portal vein (arrows) and inserting the catheter into the portal vein, (c) removing the liver out of abdominal cavity and perfusing the livers with 1% SDS and 1% Triton X-100, and (d) obtaining the decellularized livers after 1x PBS perfusion. (B) H & E staining of control (uncellularized, a) and decellularized livers (b). (C) Trichrome staining of control (a) and decellularized liver tissues (b). Representative resulst are shown. (D) Quantitative determination of the total DNA content of the control and decellularized liver tissues (n=5).