Skip to main content
. Author manuscript; available in PMC: 2014 Jun 13.
Published in final edited form as: Science. 2012 Sep 7;337(6099):1222–1225. doi: 10.1126/science.1219379

Fig. 1.

Fig. 1

Rad51-II3A binds DNA to form nucleoprotein filaments. (A) Rad51-WT (closed circle) and Rad51-II3A (open-square) bind oligo-dT as revealed by fluorescence polarization. Error bars represent SEM, N = 4. The apparent affinity of both proteins is about 300 nM. (B) EMSA analysis of DNA binding. Protein (at 0, 1, 2, 4, and 6 μM) binds M13mp18 ssDNA (22 μM nucleotides) and linear pBluescript dsDNA (8 μM base pairs). The inverted images of agarose gels are presented. The percentage of DNA in Rad51-DNA complexes is plotted. SEM, N = 6 for ssDNA, N = 3 for dsDNA. (C) Rad51-WT and Rad51-II3A proteins form filaments on a 1000-nucleotide fragment of ØX174 ssDNA. (D) ssDNA-Rad51-WT but not ssDNA-Rad51-II3A filaments bind 32P-pBluescript dsDNA (18 μM base pairs). Filament concentrations = 0, 1.5, 2.9, 4.4, 5.8, and 7.3 μM protomer (at 3nt/protomer). Phorphorimage of radioactive EMSA gel is presented. For plotted data, N= 3.