Fig. 2.

Time course of effect of calorie restriction on fuel selection pattern. AL-fed mice were placed in metabolic cages before starting a CR diet (A) and again on days 1 (B), 4 (C), 7 (D), 28 (E), and 56 (F) of calorie restriction. Gas exchange was monitored for 24 h each time mice were placed in metabolic cages. Respiratory exchange ratios (RER) were recorded 6 times/h and then averaged to give RER for each hour. Values are means ± SE for 4 mice/diet. ↓Time when daily food was provided to CR mice.