Fig. 7.

Effect of calorie restriction on lipogenic gene expression in adipose tissue. FAS (A), ACC1 (B), SREBP-1 (C), and PPARγ (D) gene expression was measured in the adipose tissue of CR and AL mice at 6 PM, 9 PM, or 12 AM. Expression was normalized first to both GAPDH and β2-microglobulin, and then these normalized values were averaged. Values are shown relative to AL values measured at 6 PM (RU). Values are means ± SE (n = 6 mice/dietary group). ***P < 0.001, difference from time-matched AL values by ANOVA and Bonferroni post hoc test. ↓Time when daily food was provided to CR mice.