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Preliminary in vitro validation of hit compounds on hα4β2 and hα3β4 nAChRs. The hit molecules, identified through structure-based VS, were validated using 50 μM concentrations on both hα4β2 and hα3β4 nAChRs, using the calcium accumulation assay. Values shown are meana ± SEMs of threeexperiments, performed in triplicate. Results are expressed as percentage of control, epibatidine-stimulated peak fluorescence levels.
