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Concentration–response studies of hit molecules on hα4β2 and hα3β4 nAChRs. Hit compounds 1, 2, 4, and 9 were tested on both hα4β2 and hα3β4 nAChRs at the indicated concentrations, using the calcium accumulation assay. Values shown are means ± SEMs of 3–10 experiments, performed in triplicate. Results are expressed as percentage of control, epibatidine-stimulated peak fluorescence levels.
