. 2014 Jun 3;118(23):6049–6061. doi: 10.1021/jp500825x

Table 1. Kinetic and Activation Parameters for the ht-ADH Single-Trp Variants^a.

mutant	k_cat (s^–1)	K_m(NAD⁺) (mM)	K_m(d-BnOH) (mM)	^Dk_cat/^D(k_cat/K_M), NAD⁺	^Dk_cat/^D(k_cat/K_M), BnOH	KIE^c	ΔH^‡(lo)−ΔH^‡(hi), (kcal/mol)^d
WT^b	8.0 (1.0)	1.1 (0.1)	3.8 (0.5)	1.0 (0.1)	0.8 (0.2)	3.1 (0.2)	6.7 (0.9)^e
W87in	3.4 (0.2)	1.8 (0.2)	4.6 (0.6)	1.0 (0.2)	1.0 (0.2)	2.5 (0.1)	5.4 (0.4)
W167in	3.0 (0.2)	1.3 (0.3)	6.7 (0.4)	0.8 (0.1)	0.9 (0.1)	2.2 (0.1)	5.7 (0.4)

All kinetic parameters are reported at 30 °C.

From ref (65).

Defined as k_cat(H-BnOH)/k_cat(D-BnOH).

ΔH^‡(lo) indicates ΔH^‡ ≤ 30 °C; ΔH^‡(hi) indicates ΔH^‡ ≥ 30 °C.

ΔH^‡(lo) = 22, 18.9, and 19 kcal/mol for WT, W87in, and W167in, respectively.

Table 1. Kinetic and Activation Parameters for the ht-ADH Single-Trp Variantsa.