Skip to main content
. Author manuscript; available in PMC: 2014 Jun 13.
Published in final edited form as: J Ocul Biol. 2013 Jun 5;1(1):0002. doi: 10.13188/2334-2838.1000002

Figure 6.

Figure 6

Regulation of Reactive Oxygen Species (ROS) generation in HTM cells by Rac GTPase. Intracellular ROS generation in TM cells was quantified by flow cytometry using the fluorogenic substrate H2DCFDA. Serum starved HTM cells (3×106 cells/10 cm petri plate) stimulated with PDGF (10 ng/ml for 4 h) (A) or expressing the constitutively active Rac GTPase (B) exhibited significant induction of ROS generation. This response was suppressed when cells were pretreated with either DPI (2 µM for 2 h; inhibitor of NADPH oxidase) orwith NAC (1 mM for 2 h; antioxidative agent). Additionally, the Rac GTPase inhibitor EHT1864 or the expression of dominant negative Rac GTPase reduced ROS generation in HTM cells, as compared to untreated controls. Similarly, DPI and NAC both decreased the ROS production in HTM cells significantly compared to untreated cells. (*denotes statistical significance at p<0.05 based on the mean values of 3 independent experiments). Error bars denote standard error.