Table 1. Culture conditions for the four prymnesiophyte species in this study.
Species | Media | Temp | L:D cycle | Irradiance |
Prymnesium parvum | L1 –silica, 18 ppta | 18°C | 12:12 | 300 µE·m−2 s−1 |
Chrysochromulina brevifilum | Modified F/2b, 30 ppt | 18°C | 12:12 | 300 µE·m−2 s−1 |
Chrysochromulina ericina | LKSc –silica, 36 ppt | 18°C | 12:12 | 300 µE·m−2 s−1 |
Phaeocystis antarctica | LKSc –silica, 36 ppt | 1°C | 12:12 | 300 µE·m−2 s−1 |
Salinity is indicated as parts per thousand (ppt).
Modified F/2 contains the following: NaNO3 2.33 mM; Na2HPO4 0.067 mM; No silica; Soil extract; L1 Trace Metals; F/2 vitamins.
LKS media is a combination of L1 and K media and soil extract (https://ncma.bigelow.org/algal-recipes).