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. 2014 Jun 13;9(6):e97911. doi: 10.1371/journal.pone.0097911

Figure 1. (a,b,c & d)- PCR of of LdH2B, LdH3, LdH4 and LdH2A genes.

Figure 1

(e, f, g, & h)- Expression and purification of LdH2B, LdH3, LdH2A, LdH4 from E. coli rossetta cells, WCL of transformed E. coli separated on 12% acrylamide gel and stained with Coomassie blue, M: Molecular wt. markers; Lane 1: Flow through; lane 2&3: Washing fraction; Lane 4 & 5: eluted protein. (i, j, k & l)-Western blot analysis using anti-rLhistone antibody in uninduced WCL, induced WCL and leishmania WCL - M: Mol wt marker, Lane 1: uninduced WCL, Lane 2: induced WCL, Lane 3: Purified protein, Lane 4: whole cell lysate of Leishmania.