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. 2014 Jun 13;9(6):e97911. doi: 10.1371/journal.pone.0097911

Figure 2. Cellular responses of rLdH2B, rLdH3, rLdH2A, rLdH4, and pooled(H2-4) of L.donovani in hamsters.

Figure 2

(a) XTT response of mononuclear cells (lymph nodes) from normal, L.donovani infected (30 day p.c.) and treated hamsters in response to Con A, SLD and rLdH2B, rLdH3, rLdH2A, rLdH4 and pooled (rLdH2-4) at 10 µg/ml each. Proliferation was represented as mean OD of stimulated culture-mean OD of unstimulated control. Each bar represents the pooled data (mean ± S.D. value) of 6 hamsters and the data represent the means of triplicate wells ± the S.D. (b) Nitric oxide production (µM) by the J774 A.1 cells were primed with the supernatants of stimulated lymphocytes (3 days with mitogen and 5 days with antigens) of normal, infected and cured hamsters in response to rLdH2B, rLdH3, rLdH2A, rLdH4 and pooled (H2-4), SLD and LPS respectively at 10 µg/ml each. The estimation of NO production was done using Greiss reagent in supernatants collected from macrophage cultures 24 h after incubation and the absorbance of the reaction product was measured at 540 nm. Significance values indicate the difference between the SLD and rLdH2B, rLdH3, rLdH2A, rLdH4, and pooled (H2-4) stimulation (**, p<0.01; ***, p<0.001).