Fig. 3.
A chain extension and ketoreduction cycle with a fluorinated extender using a simple polyketide synthase, NphT7. (A) Reactions catalyzed by NphT7 and PhaB. (B) Steady-state kinetic parameters for NphT7-catalyzed C–C bond formation measured using a coupled assay with PhaB. Data points are reported as the mean ± s.d. (n = 3). Kinetic parameters are reported as mean ± s.e. (n = 3) as determined from non-linear curve-fitting. Error in the kcat/KM parameter was obtained from propagation of error from the individual kinetic terms.