Table 2.
CD4 + T cells (CD3 + , CD4 + , FOXP3 – ) gained BTLA expression (MFI) 72 hours following CLP surgery
| Sham mice (n = 6) | CLP mice (n = 10) | P value | |
|---|---|---|---|
| Blood – 24 hours post surgery |
|
|
|
| % BTLA+ (SEM) |
96.72 (0.65) |
93.36 (0.88) |
0.01 |
| BTLA MFI (SEM) |
11.91 (1.07) |
10.00 (0.41) |
NS |
| Blood – 72 hours post surgery |
|
|
|
| % BTLA+ (SEM) |
95.80 (1.41) |
94.34 (1.70) |
NS |
| BTLA MFI (SEM) |
8.79 (0.48) |
12.10 (0.66) |
0.02 |
| Spleen – 24 hours post surgery |
|
|
|
| % BTLA+ (SEM) |
97.67 (0.27) |
97.29 (0.50) |
NS |
| BTLA MFI (SEM) |
10.89 (0.20) |
10.73 (0.76) |
NS |
| Spleen – 72 hours post surgery |
|
|
|
| % BTLA+ (SEM) |
98.44 (0.36) |
98.23 (0.90) |
NS |
| BTLA MFI (SEM) | 7.43 (0.18) | 11.64 (0.64) | <0.01 |
P value evaluated via an unpaired, two-tailed Student’s t test ± standard error of the mean (SEM); bold values are significant. Mean fluorescent intensity (MFI) experiments were conducted with three to five mice for each group and then repeated for confirmation of the results. BTLA, B and T lymphocyte attenuator; CLP, cecal ligation and puncture; NS, not significant.