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. 2014 Jun 13;9(6):e99625. doi: 10.1371/journal.pone.0099625

Figure 4. CRISPLD2 regulates the response to inflammatory cytokines.

Figure 4

A) Effect of CRISPLD2-specific siRNA on CRISPLD2 mRNA and protein levels. ASM cells were transfected with CRISPLD2-specific siRNA or non-targeting (NT) siRNA, and 72 h later, CRISPLD2 mRNA and protein levels were determined by qRT-PCR (levels normalized to those in control cells transfected with NT siRNA) and immuno-blotting, respectively. The effect of CRISPLD2 knockdown on IL1β-induced cytokine expression was assessed by transfecting ASM cells with CRISPLD2-specific or NT siRNA, and 72 h later, treating cells for 24 h with B) 5 ng/mL IL1β, C) 100 nM DEX, or D) 5 ng/mL IL1β and 100 nM DEX. IL6 expression was determined by qRT-PCR. Normalized mRNA levels are shown. Experiments were performed in triplicate, and the error bars are SE values for three samples. * P<0.05 (t test).