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. 2014 Jun 13;9(6):e99245. doi: 10.1371/journal.pone.0099245

Figure 5. Direct regulation of SREBP-1c through PPARα was indispensable in PPARα-induced liver triglyceride accumulation.

Figure 5

(A) PPARα-dependent regulation of the SREBP-1c promoter was analyzed through luciferase assays using the human SREBP-1c-1564/+1-luc construct in the presence of the indicated amounts of fenofibrate for 24 h. The activities of the constructs transfected into HepG2 cells under the indicated dosages are shown. The results represent relative Firefly/Renilla luciferase activities compared with the wild-type construct under basal conditions as the reference value. (B) HepG2 cells were transfected with the empty plasmid pcDNA3.1 or DN-SREBP-1c and subsequently treated with or without 50 µM fenofibrate in serum-free medium for an additional 48 hours. The cells were collected and assayed to determine the intracellular TG content. The content was normalized to the total protein from the same group. The data were calculated from 3 independent experiments and are expressed as the mean ± SD. * P<0.05 versus the control group. # P<0.05 versus the fenofibrate 10 µM group.