Table 1. Primers used for the analysis of mRNA expression levels in humans.
Gene | NM | Product (bp) | Forward primer | Reverse primer |
Srebp1c | NM_001005291.2 | 80 | GGAGGGGTAGGGCCAACGGCCT | CATGTCTTCGAAAGTGCAATCC |
PPARα | NM_001001928.2 | 304 | AAGGGCTTCTTTCGGCGAAC | TGACCTTGTTCATGTTGAAGTTCTTCA |
SCD1 | NM_005063.4 | 281 | CCTCTACTTGGAAGACGACATTCG | GCAGCCGAGCTTTGTAAGAGC |
FASN | NM_004104.4 | 159 | CGGAAACTGCAGGAGCTGTC | CACGGAGTTGAGCCGCAT |
ACC | NM_198834.1 | 253 | GAATGTTTGGGGATATTTCAG | TTCTGCTATCAGTCTGTCCAG |
Cpt1α | NM_001031847.2 | 133 | CCTCCAGTTGGCTTATCGTG | TTCTTCGTCTGGCTGGACAT |
Srebp1a | NM_001005291.2 | 135 | ATGGACGAGCCACCCTTC | GCCAGGGAAGTCACTGTCTTG |
FABP1 | NM_002080.2 | 142 | ATCCCACGGGAGTGGACCCG | CGCACAGCCCAGGCATCCTT |
ApoB | NM_000384.2 | 107 | CAACCCTGAGGGCAAAGCCTTGCTG | CCTGCTTCCCTTCTGGAATGGCC |
DGAT2 | NM_001253891.1 | 215 | TGGGGGCTGGTGCCCTACTC | AATTGGCCCCGAAGGCTGGA |
GPAT1 | NM_001244949.1 | 154 | AACCCCAGTATCCCGTCTTT | CAGTCACATTGGTGGCAAAC |
β-actin | NM_001101.3 | 104 | ACAGAGCCTCGCCTTTGCCG | ACATGCCGGAGCCGTTGTCG |