Table 2. Primers used for the analysis of mRNA expression levels in mice.
Gene | NM | Product (bp) | Forward primer | Reverse primer |
Srebp1c | NM_011480.3 | 113 | GCGCTACCGGTCTTCTATCA | GGATGTAGTCGATGGCCTTG |
PPARα | NM_001001928.2 | 304 | AAGGGCTTCTTTCGGCGAAC | TGACCTTGTTCATGTTGAAGTTCTTCA |
SCD1 | NM_009127.4 | 242 | AAGATATTCACGACCCCACC | CAGCCGTGCCTTGTAAGTTC |
FASN | NM_007988.3 | 234 | GTCCTGGGAGGAATGTAAACAG | CGGATCACCTTCTTGAGAGC |
ACC | NM_133360.2 | 235 | GCTTATTGATCAGTTATGTGGCC | CTGCAGGTTCTCAATGCAAA |
Cpt1α | NM_013495.2 | 100 | TTGGGCCGGTTGCTGAT | GTCTCAGGGCTAGAGAACTTGGAA |
Srebp1a | NM_011480.3 | 69 | GGCCGAGATGTGCGAACT | TTGTTGATGAGCTGGAGCATGT |
FABP1 | NM_017399.4 | 74 | TCAAGCTGGAAGGTGACAATAA | GTCTCCATTGAGTTCAGTCACG |
ApoB | NM_009693.2 | 121 | AAACATGCAGAGCTACTTTGGAG | TTTAGGATCACTTCCTGGTCAAA |
DGAT2 | NM_026384.3 | 66 | AGAACCGCAAAGGCTTTGTG | AGGAATAAGTGGGAACCAGATCAG |
GPAT1 | NM_008149.3 | 67 | CAACACCATCCCCGACATC | GTGACCTTCGATTATGCGATCA |
β-actin | NM 007393.3 | 101 | ACCCCAGCCATGTACGTAGC | GTGTGGGTGACCCCGTCTC |