Fig. 4.

PRKD2 silencing upregulates expression of tumor suppressor proteins. U87MG and GM133 cells were transfected with nontargeting (S) and PRKD2 targeting siRNA (P5), and expression analysis of tumor suppressor proteins was performed by immunoblotting in cytosolic and nuclear fractions 3 days post transfection. Untreated cells (“C”) were used as controls. Nuclear and cytoplasmic lysates were analyzed for expression of PRKD2, p53, p21, p27, and p15. Lamin A/C, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or β-actin was used as loading controls.