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. 2014 Jun 13;9(6):e99586. doi: 10.1371/journal.pone.0099586

Figure 6. Effect of LY294002(LY) or U0126(U) on the anti-apoptotic activity of TCDD.

Figure 6

Beas2B cells were pretreated with 10 µM LY or 100 nM U for 1 h, then treated with TCDD (10 nM) for 4 h, followed by 100 nM STS for 2 h. (A) The expression of p-ERK1/2, ERK1/2, p-Akt, and Akt in different treatment groups was determined by western blotting analysis. Representative data from one of three independent experiments are shown. (B) The level of apoptosis after treatment with LY or U was measured by Annexin V staining and analyzed by flow cytometry, and the quantified results are shown (C). The data represent the mean ± SEM of three independent experiments; *p<0.05 compared with the control (Con) group; #p<0.05 compared with the STS-treated group, †p<0.05 compared with the TCDD + STS group.