Table 1. The number of valid protein identifications and significantly differentially expressed genes and proteins after temperature downshift of E. coli O157∶H7 Sakai from 35°C to 14°C.
| Time points (min) | Number of biological replicates | Number of proteinsb | Number of differentially expressed genes and proteinsd | |||||
| Transcriptome | Proteomea | Membrane fraction | Soluble fraction | Total fraction | Trancriptome (total)c | Proteome (total) | Transcriptome vs. Proteomee | |
| Before downshift | 3 | 6(6) | 343 | 1,320 | 1,370 | NAe | NA | NA |
| 30 min after the shift | 3 | 2(2) | 704 | 824 | 1,074 | 503(292) | 137(20) | 13(3) |
| 90 min after the shift | 3 | 2(2) | 717 | 864 | 1,107 | 637(466) | 155(26) | 26(2) |
| 160 min after the shift | 3 | 2(2) | 702 | 890 | 1,115 | 594(378) | 118(25) | 22(6) |
| 330 min after the shift | 3 | 2(2) | 858 | 864 | 1,185 | 496(301) | 201(63) | 30(7) |
a
Number of proteins identified in soluble (outside brackets) and membrane (within brackets) fractions of the proteome that have passed the filtering criteria.
b
Number of protein identifications with high confidence in membrane, soluble and total fractions of the proteome.
c
Analysis does not include the number of differentially expressed undefined intergenic regions in the transcriptome.
d
Number of up-regulated (outside brackets) and down-regulated (within brackets) genes and proteins.
e
Number of genes and proteins significantly up- or down-regulated in both the transcriptomic and proteomic analysis.
e
NA; not applicable.