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. 2014 Jun 13;9(6):e98966. doi: 10.1371/journal.pone.0098966

Figure 6. GSK-3 silencing dramatically affects adult A. fluviatilis engorgement and development.

Figure 6

Unfed Wol+ females were injected with 2 µg of either unrelated double-stranded RNA (β-gal, dashed bar) or double-stranded RNA specific for GSK-3 (black bar) and were fed on blood 24 h after injection. RNA was extracted three days after the blood meal to confirm silencing. After further 3 days, the width of the mosquito's abdomen was measured in both groups under a stereomicroscope. (B) Photos representing the graph are shown in A. (C) Confirmation of silencing by real-time PCR. The results are expressed as the mean ± S.E. of three independent experiments conducted in triplicate (Paired T test, p value <0.05 was considered statistically significant).