Figure 3. The GAS5-derived snoRNAs are not suitable housekeeping genes for use in experiments where DOX treatment.

Relative levels of miR-34a normalised to (A) U6 snRNA, (B) U47 snoRNA, (C) U44 snoRNA and (D) U19 snoRNA were measured by RT-qPCR in p53^WT HCT116 cell lines and p53^KO HCT116 cell lines treated with either doxorubicin (at a final concentration 0.2 ug/ml) or vehicle for 24 hours. Data are presented relative to the vehicle treated cells ± s.e.m (each of them performed in triplicate; Student's t test: *P<0.01, **P≤0.006).