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. 2014 Jun 13;9(6):e98561. doi: 10.1371/journal.pone.0098561

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© 2014 Krell et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A & B) Western blot's showing p53 levels in the first 10 normal colorectal (A) and colorectal tumour (B) tissue samples. GAPDH was used as a loading control. (C) Column chart demonstrating the fold changes in p53 expression levels shown in the Western blots (A & B) normalised to GAPDH and calculated using ImageJ software. (D) A box plot comparing the relative expression levels of p53 between all 25 paired colorectal tumour (T) and normal colorectal (N) tissue samples (Student's t test *P<0.01).