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Summary of experimentally estimated pKa values using the respective kcat/Km and kcat (within parenthesis) profiles.
| sPBP 6 Species | Acidic Limb pKa | Basic Limb pKa |
|---|---|---|
| Wild-typea | 8.3 (8.4), 9.0 (9.4) | 11.1 (11.4) |
| γ-thialysine69b | 8.1 (8.7) | 10.4 (10.3) |
| γ-thialysine235b | 8.4 (8.9) | 10.1 (10.0) |
Fit to the triple ionization model.
Fit to the double ionization model.
