Figure 5.

TP53 mutational status corresponds to p53 protein accumulation. (A) Case 939 showed a heterogeneous pattern of staining, which is consistent with TP53 mutational status (21% of mutated alleles, I195N). (B) Case 108, with no evident p53 labelling, corresponds to a homozygous stop mutation (83% of mutated alleles, R306*). For each sample, a representative p53 immunohistochemical image (original magnification = ×20) and a representation of the reads aligned to the reference genome, as provided by the Integrative Genomics Viewer software (IGV v 2.1, Broad Institute), are presented.