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. Author manuscript; available in PMC: 2014 Dec 12.
Published in final edited form as: Nature. 2014 Apr 6;510(7504):241–246. doi: 10.1038/nature13120

Extended Data Figure 7. Effects of dmc1 deletion or spo11 hypomorphic mutation on zmm mutant phenotypes.

Extended Data Figure 7

a, b, ZMM status is irrelevant in a dmc1 background. Broken chromosomes accumulate to similar levels in a dmc1 single mutant and dmc1 zmm double mutants. Representative pulsed-field gel Southern blots probed for Chr IX are in a and Poisson-corrected quantification of DSBs is in b (mean ± SD, 3 cultures). P, parental; W, wells. c, Reducing Spo11 activity in a zip3 mutant partially alleviates the prophase I delay/arrest. Meiotic progression was assessed by staining with DAPI and measuring the percentage of cells that had completed MI (±MII). Data are means ± SD for 3 cultures, except wild type and spo11-HA, each analyzed once.