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. Author manuscript; available in PMC: 2014 Dec 12.
Published in final edited form as: Nature. 2014 Apr 6;510(7504):241–246. doi: 10.1038/nature13120

Extended Data Figure 4. Analysis of recombination at three natural DSB hotspots.

Extended Data Figure 4

a, b, Recombination reporters at the ERG1 (a) and GAT1 (b) hotspots. c, d, e, Representative Southern blots of parental and recombinant DNA molecules at CCT6 (c), ERG1 (d), and GAT1 (e). The arrowhead in e indicates a non-reproducible radiolabeled species. f, Local distribution of DSBs around recombination reporter locations is not altered in zip3 mutants. Spo11-oligo profiles (averages for wild type and zip3) are smoothed with 201-bp Hann window; zip3 values are offset to separate profiles.