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. Author manuscript; available in PMC: 2014 Dec 12.
Published in final edited form as: Nature. 2014 Apr 6;510(7504):241–246. doi: 10.1038/nature13120

Extended Data Figure 5. Direct analysis of DSB formation at natural hotspots.

Extended Data Figure 5

Representative Southern blots of DNA separated on a conventional agarose gel and probed for GAT1 (a), CCT6 (b, c), and ERG1 (d). JMs, joint molecules; P, parental-length DNA; W, wells. The arrowhead in a indicates signal from the CCT6 parental band that remained after stripping and reprobing for GAT1. e, Quantifications for b, c, d (mean ± SD for 3 cultures).