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. 2014 Jun 7;9:2791–2801. doi: 10.2147/IJN.S58685

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© 2014 Pascolo et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Chemical imaging using Fourier transform infrared spectroscopy on single cells. (A, E) Light microscope images of a control cell (square dimension, 42×63 μm) and a cell treated with nanoparticles, respectively. (E) Box delineates the area analyzed by Fourier transform infrared spectroscopy (square dimension, 42×63 μm). Chemical imaging for the presence of lipids, proteins (amide I), and poly-lactide-co-glycolide are shown in a control cell (B–D) and in a cell incubated with poly-lactide-co-glycolide iron oxides nanoparticles (F–H). (I) Merge image of the visible light and Fe map from X-ray fluorescence analyses of the cell (E), revealing the iron oxide presence. The red box indicates the area analyzed by Fourier transform infrared spectroscopy, whereas the black one indicates the X-ray fluorescence area.