Figure 3. Effect of 810-nm laser on intracellular calcium release in cortical neurons with excitotoxicity.
(A) Fluo-4 AM (green) fluorescence for calcium and Hoechst (blue) fluorescence for nuclei in cortical neurons as control and with 3 excitotoxins, with and without LLLT (3 J/cm2 810 nm laser). Scale bar = 20 µm. (B) Quantification by Image J of mean fluorescence values. N= 6 fields per group. Error bars are SEM and * = p <0.05; ** = p<0.01; *** = p<0.001 vs sham (dark).