Figure 5. Effect of 810-nm laser on intracellular reactive oxygen species (ROS) in cortical neurons with excitotoxicity.
(A) CellRox Red (red) fluorescence for cytoplasmic ROS and Hoechst (blue) fluorescence for nuclei in cortical neurons as control and with 3 excitotoxins, with and without LLLT (3 J/cm2 810 nm laser). Scale bar = 20 µm. (B) Quantification by Image J of mean fluorescence values. N= 6 fields per group. Error bars are SEM and * = p <0.05 vs sham (dark).