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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Toxicol Lett. 2014 Apr 15;228(1):56–66. doi: 10.1016/j.toxlet.2014.04.001

Figure 6.

Figure 6

Direct toxicity of bile acids in mouse hepatocytes. LCA (A), TLCA (B), TCA (C) and TCDCA (D) were applied to primary murine hepatocytes 3h after plating at the indicated concentration for 6 or 24h. In addition, a mixture of bile acids including TLCA, TCDCA, and TCA (Bile Mix) was applied to cells at the concentrations measured at 96 h post LCA feeding to recapitulate how cells would respond to the total biliary mixture (Figure 5). These bile acids were chosen as they account for 99% of the total biliary mix. This mixture was applied for 6 h or 24 h. Cell death was assessed by LDH release. Data represent means ± SE of n = 3 individual cell isolation experiments..*P<0.05 (compared to vehicle control).