Table 1.
Gene Changes after LCA Feeding
| Inflammatory Markers (Gene) | Control | 24 h LCA | 48 h LCA | 72 h LCA | 96 h LCA |
|---|---|---|---|---|---|
| MIP2 | 1 ± 0.1 | 7.4 ± 1.2* | 6.0 ± 0.6* | 27.4 ± 9.3* | 46.4 ± 7.1* |
| mKC | 1 ± 0.4 | 2.8 ± 1.1* | 1.2 ± 0.2 | 4.9 ± 3.0* | 5.1 ± 1.2* |
| TSP-1 | 1 ± 0.4 | 24.5 ± 2.2* | 19.3± 6.8* | 23.7± 3.5* | 1.6 ± 0.5 |
| IL-1β | 1 ± 0.1 | 2.0 ± 0.5* | 1.9 ± 0.2 | 2.5 ± 0.7* | 4.4 ± 0.3* |
| IL-6 | 1 ± 0.1 | 3.8 ± 0.9* | 3.8 ± 0.5* | 7.5 ± 1.5* | 4.6 ± 1.9* |
| IL-10 | 1 ± 0.2 | 11.8 ± 3.1* | 9.1 ±1.0* | 15.8 ± 2.3* | 0.9 ± 0.4 |
| iNOS | 1 ± 0.3 | 56.0 ± 12.4* | 35.9 ± 9.8* | 65.2 ± 9.3* | 3.7 ± 1.0* |
| TNF-α | 1 ± 0.1 | 0.9 ± 0.2 | 0.7 ± 0.1 | 1.9 ± 0.3* | 3.1 ± 0.3* |
| TNFR1 | 1 ± 0.2 | 2.2 ± 0.4* | 1.8 ± 0.6 | 2.8 ± 0.4* | 1.2 ± 0.1 |
| Cox2 | 1 ± 0.4 | 2.0 ± 0.3* | 2.0 ± 0.2* | 3.8 ± 0.5* | 1.2 ± 0.1 |
| VCAM-1 | 1 ± 0.1 | 0.8 ± 0.1 | 0.5 ± 0.1 | 1.2 ± 0.6 | 1.7 ± 0.1* |
| Acute Phase Proteins | |||||
| HO-1 | 1 ± 0.2 | 1.5 ± 0.1 | 1.5 ± 0.1 | 2.3 ± 0.3* | 2.6 ± 0.5* |
| hsp86 | 1 ± 0.1 | 1.2 ± 0.2 | 0.9 ± 0.2 | 0.9 ± 0.1 | 0.4 ± 0.8 |
| Gpx1 | 1 ± 0.1 | 0.8 ± 0.1 | 1.0 ± 0.2 | 0.5 ± 0.7 | 0.4 ± 0.5 |
| MT-1 | 1 ± 0.1 | 2.0 ± 0.8 | 6.2 ± 3.0* | 55.8 ± 9.4* | 73.1 ± 24.7* |
RT-PCR was used to assess gene changes after LCA feeding. Data represent means ± SE of n=3 animals per group and time points.
*
P<0.05 (compared to controls).
ICAM-1, intercellular adhesion molecule-1, iNOS, inducible nitric oxide synthase; mKC, mouse keratinocyte chemoattractant; IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor α; TNFR1, tumor necrosis factor alpha receptor 1; Cox-2, cyclooxygenase-2; VCAM-1, vascular adhesion molecule-1; HO-1, heme oxygenase-1; hsp86, heat shock protein-86; Gpx-1, glutathione peroxidase-1; MT-1, metallothionein-1; MIP-2, macrophage inflammatory protein 2; TSP-1, thrombospondin 1;