Table 1. Serum concentrations of Vitamin D and weights of mice in the two dietary groups after dietary restriction.

weeks	two		four		six		eight
	VitD+	VitD-	VitD+	VitD-	VitD+	VitD-	VitD+	VitD-
25-(OH)D3 (ng/ml)	43.4±0.67	22.3±0.89***	63.2±1.02	12.3±0.32***	75.6±1.21	8.56±0.21***	82.04±1.74	5.1±0.25***
Weight(g)	16.8±0.38	16.7±0.26	18.3±0.26	18.7±0.28	19.9±0.27	19.2±0.2	20.65±0.17	20.75±0.16

C57BL/6 mice were raised on VitD+ and VitD- diets from weaning. Every two weeks thereafter, three mice from each group were bled from the retro-orbital venous plexus and the serum concentrations of 25-(OH)D3(ng/ml) were analyzed. Values represent mean±SE (n = 3,repeated three times each). Statistical significance between the two groups of mice was evaluated using a Student's t-test p<0.001(***).