Table 1. PCR primers and probes.
Primer | Sequence 5′-3′ |
TdT-FW | GCTTCAGGTACAGAACATA |
TdT-REV | GTCTGTTCTCACAACAAG |
TdT probe | FAM-ACT[+C]CT[+T]GA[+T]GT[+C]TCCTG-BHQ1* |
T7 | TAATACGACTCACTATAGGG |
T3 | ATTAACCCTCACTAAAGGGA |
TdT_tr1_1925_FW | AGACCAAGTGCACATATG |
TdT_tr1_1925_PR | FAM-ATTTCTTCTTCACTTTCCGCTTTGAGA-BHQ1 |
TdT_tr1_1925_RV | GGTTCAATGTAGTCCAATC |
TdT_tr2_131_FW | TGGTCAGGTTTTGGATTTC |
TdT_tr2_131_PR | HEX-CAGAAATGCCCACACAGCCTC-BHQ1 |
TdT_tr2_131_RV | CCTGTCATGGTGACAAAG |
18S fwd | TGGTTGCAAAGCTGAAACTTAAAG |
18S probe | HEX-CCTGGTGGTGCCCTTCCGTCA-BHQ1 |
18S rev | AGTCAAATTAAGCCGCAGGC |
IgLg1fwd | GGCCCAGGCTGTGCTGACTC |
IgLg1 rev | TGATGGTGCTGCCGTCTGCC |
IgKg2 fwd | TGTGCTGACCCAGACTCCCCT |
IgKg2 rev | ACAGTTCCGGTCTTCAGCTGCTC |
IgH fwd1 | TTGTGCTSTCAGCCCCCAGA |
IgH rev1 | CGCAGGACACCAGGGGGAAG |
IGHV fwd3 | GGACAACTCCAAGAGCCAAG |
IGHJrev2-FAM | TGAGGAGACGGTGACCMKGAG |
*Nucleotides in square brackets refer to locked nucleic acids.