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. 2014 Jun 13;9(6):e99808. doi: 10.1371/journal.pone.0099808

Table 1. PCR primers and probes.

Primer Sequence 5′-3′
TdT-FW GCTTCAGGTACAGAACATA
TdT-REV GTCTGTTCTCACAACAAG
TdT probe FAM-ACT[+C]CT[+T]GA[+T]GT[+C]TCCTG-BHQ1*
T7 TAATACGACTCACTATAGGG
T3 ATTAACCCTCACTAAAGGGA
TdT_tr1_1925_FW AGACCAAGTGCACATATG
TdT_tr1_1925_PR FAM-ATTTCTTCTTCACTTTCCGCTTTGAGA-BHQ1
TdT_tr1_1925_RV GGTTCAATGTAGTCCAATC
TdT_tr2_131_FW TGGTCAGGTTTTGGATTTC
TdT_tr2_131_PR HEX-CAGAAATGCCCACACAGCCTC-BHQ1
TdT_tr2_131_RV CCTGTCATGGTGACAAAG
18S fwd TGGTTGCAAAGCTGAAACTTAAAG
18S probe HEX-CCTGGTGGTGCCCTTCCGTCA-BHQ1
18S rev AGTCAAATTAAGCCGCAGGC
IgLg1fwd GGCCCAGGCTGTGCTGACTC
IgLg1 rev TGATGGTGCTGCCGTCTGCC
IgKg2 fwd TGTGCTGACCCAGACTCCCCT
IgKg2 rev ACAGTTCCGGTCTTCAGCTGCTC
IgH fwd1 TTGTGCTSTCAGCCCCCAGA
IgH rev1 CGCAGGACACCAGGGGGAAG
IGHV fwd3 GGACAACTCCAAGAGCCAAG
IGHJrev2-FAM TGAGGAGACGGTGACCMKGAG

*Nucleotides in square brackets refer to locked nucleic acids.