Table 1.
Absolute values for miR expression (no unit) and protein abundance of MMPs and TIMPs (pg/ml) in aortic tissue from normal and TAA patients with BAV or TAV.
| TISSUE ANALYTE | CONTROL | BAV | TAV |
|---|---|---|---|
| microRNA (2−ΔCt) | |||
| miR-1 (1X10−3) | 57.8±2.4 | 54.3±3.3 | 39.4±3.3*# |
| miR-21 (1X10−2) | 169.7±21.4 | 217.7±32.3 | 683.0±191.0*# |
| miR-29a (1X10−2) | 242.4±33.3 | 274.7±17.2 | 220.2±25.8 |
| miR-133a (1X10−3) | 80.3±5.2 | 81.7±5.8 | 69.2±5.1 |
| miR-143 (1X10−2) | 830.9±37.9 | 703.7±45.9 | 560.6±49.1* |
| miR-145 (1X10−2) | 771.0±37.9 | 910.8±72.1 | 715.0±75.6 |
| MMPs (pg/mL) | |||
| MMP-1 | 3.4±1.0 | 15.7±4.0 | 11.3±2.8 |
| MMP-2 (1X102) | 71.6±13.0 | 64.1±7.7 | 56.9±5.4 |
| MMP-3 | 292.9±45.5 | 165.2±22.4 | 160.6±20.2 |
| MMP-7 (1X101) | 8.8±2.9 | 14.6±3.8 | 24.0±7.2 |
| MMP-8 (1X101) | 294.1±128.8 | 121.7±29.3 | 128.5±49.8 |
| MMP-9 (1X102) | 74.9±49.6 | 28.3±7.1 | 26.0±7.9 |
| MMP-12 | ND | ND | ND |
| MMP-13 | ND | ND | ND |
| TIMPs (pg/mL) | |||
| TIMP-1 (1X102) | 113.6±13.7 | 100.2±5.9 | 111.3±6.1 |
| TIMP-2 (1X102) | 127.9±17.1 | 114.2±7.5 | 116.3±6.5 |
| TIMP-3 (1X102) | 22.2±3.0 | 16.5±1.5 | 18.8±2.2 |
| TIMP-4 | 141.9±33.7 | 49.8±5.7* | 43.6±4.6* |
|
| |||
| Sample Size (n) | 10 | 21 | 21 |
*
p<0.05 vs. Control
#
p<0.05 vs. BAV
Data Analysis
All analytes were subjected to a Shapiro-Wilk test for normality. For the unequally distributed analytes, the absolute values were log transformed and subjected to an one-way analysis of variance (prcompw module) with Tukey’s wholly significant difference post-hoc analysis for separation of means to determine differences between the referent controls, BAV, and TAV groups.